منابع مشابه
Two Epstein-Barr Virus-associated DNA Polymerase
We have partially purified and characterized two separate DNA polymerase activities associated with Epstein-Barr virus (EB virus). One activity is present in EB virus producer cell lines but not in nonproducer or negative cell lines. It adheres more strongly to DEAE-cellulose than any host cell enzymes, eluting at 210 to 270 mM potassium phosphate buffer. Further elution from phosphocellulose a...
متن کاملRegulation of the Epstein-Barr virus DNA polymerase gene.
The gene (pol) encoding the Epstein-Barr virus (EBV) DNA polymerase is a member of the "early" class of viral genes which are expressed shortly after activation of latent virus infection. First, mRNA from the EBV-producing cell line, B95-8, treated with 12-O-tetradecanoylphorbol-13-acetate and sodium butyrate to induce lytic replication and expression of this gene was analyzed. Northern (RNA) a...
متن کاملSpecific immune serum to the Epstein-Barr virus DNA polymerase.
Epstein-Barr virus (EBV) DNA polymerase was released from phorbol ester-treated tamarin (Saguinus oedipus) cells (B95-8) and prepared for use as an antigen by sequential column chromatography with DEAE-Sephadex A-25, DEAE-cellulose, phosphocellulose, and single-stranded DNA cellulose. Proteins from single-stranded DNA cellulose with DNA polymerase activity in 100 mM ammonium sulfate were mixed ...
متن کاملTwo Epstein-Barr virus-associated DNA polymerase activities.
We have partially purified and characterized two separate DNA polymerase activities associated with Epstein-Barr virus (EB virus). One activity is present in EB virus producer cell lines but not in nonproducer or negative cell lines. It adheres more strongly to DEAE-cellulose than any host cell enzymes, eluting at 210 to 270 mM potassium phosphate buffer. Further elution from phosphocellulose a...
متن کاملEpstein-Barr Virus DNA Replication
Epstein-Barr virus (EBV) and its close relatives are the only known viruses that have two fully independent systems for replicating their genomes, one that supports virus production during active or "lytic" infection, and a second one that operates during latent stages of infection to allow the circular viral genomes to be duplicated during each cell division cycle. Although EBV's system of lyt...
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ژورنال
عنوان ژورنال: Uirusu
سال: 1994
ISSN: 0042-6857,1884-3433
DOI: 10.2222/jsv.44.177